Scientific Instrument

CHEETAH™ : Light Field Microscopic Add-on Module

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Detecting The Undetectable In Real-time 3D

Analyze mechanism-of-action (MOA) in depth of organotypic 3D cell culture with unprecedented volumetric frame rate (>30fps)

  • Neuronal activity
  • Cardiac/Muscular activity
  • Immune activity
  • Complex functional activity
  • Extractability of a single-shot 3D image in 32 stacks each frame and individual intensity data point

Scientific Camera Selection At Your Needs

Seamless Installation On Wide-field Fluorescent Microscope

Volumetric Dimensions For Your Interest

The System was Optimized in

CPU : AMD Ryzen Threadripper PRO 3955WX

GPU : NVIDIA RTX A5000 D6

RAM : 128 GB

CHEETAH™ : Applications

Calcium imaging of Fluo-4 Labeled HEK293 3D spheroid

Human embryonic kidney (HEK293) cells are widely used for the heterologous expression of voltage- and ligand-gated ion channels. Fluo-4, calcium ion indicator, was labeled to visualize and monitor changes in intracellular calcium levels. This video includes in observation the calcium dynamics of HEK293 spheroid in 10 fps 3D.

Dose response GPCR assay on HEK293 spheroids

  • Real-time 3D observations (10fps in 3D) and measurements to analyze how calcium indicator labeled HEK293 spheroids responding to varying doses of carbachol, an agonist that binds to muscarinic acetylcholine receptors (mAChRs), a subfamily of G-protein coupled receptors (GPCR) expressed on the cells.
  • Calcium signaling in GPCR is a critical process involving increasing the concentration of calcium ions in the cytoplasm to initiate cellular events.

Cardiotoxicity assay on iPSC-derived cardiomyocyte 3D spheroids

  • Real-time 3D observations and measurements of cardiac 3D spheroids (Only 20K cells per well) for cardiotoxicity assay, utilizing NEXEL’s Cardiosight®-S in calcium imaging and voltage imaging with an interval time of 33ms and 11ms respectively.
  • By combining calcium imaging’s prowess in tracking cellular activity with voltage imaging’s ability to monitor membrane potential, we gained unprecedented insights into the electrophysiological behavior of 3D cardiac miniatures with different doses of drug, potassium channels blocker, E-4031.